Stunner

Overview

Stunner is the only system that pulls together UV/Vis concentration, dynamic light scattering (DLS) and static light scattering (SLS) data from the same 2 μL sample.
Dig in to your AAV to get the total capsid titer and empty/full ratio, or rack up payload concentration and size data on any nanoparticle all at once.
Nail down your protein quality by knocking concentration, hydrodynamic size, polydispersity, and detection of aggregates off your list in one shot.
Without skipping a beat, you’ll know if your AAV, nanoparticle or protein is good to go.


AAV capsid titer
AAV empty/full ratio
LNP total RNA quant
Nanoparticle payload quant
Aggregation
Concentration
Sizing & polydispersity
B22 & kD

  • Capabilities
  • Specifications
  • Teeny sample, tons of information

Just load 2 μL of sample in a Stunner plate — don’t bother with sample prep or dilution. Each microfluidic circuit has two fixed pathlengths built into it to cover a wide dynamic range of 0.03-275 OD. If you’re dealing with a full plate, get 96 concentration measurements in 10 minutes — add on DLS sizing and have both done in an hour. For even heavier workflows, hook it up to your favorite robot to add more oomph

  • Look your AAV up and down

Drop in your AAV and before you can blink, DLS & SLS figure out how many intact capsids you have or if a bunch of aggregates are screwing things up. See empty/full ratio, total protein and total ssDNA in about a minute with UV/Vis. Don’t worry about extinction coefficients or overlapping spectra – Stunner does all the math for you.

  • Know your AAV inside out

Get to the numbers you actually want - titers. Stunner bridges DLS and UV/Vis data to tally up how many full and empty capsids are present, and how much extra protein and DNA is left over. Take your cleaned up AAV and sneak a peek down to 1012 vg/mL. In just one assay, Stunner’s dye-free, label-free, standard-free, hassle-free workflow tells the whole titer story.

  • Downstream ready

Smash through the USP & Ph. Eur. UV/Vis requirements for absorbance accuracy, precision, linearity, wavelength accuracy, stray light and resolution with performance verification measurements of independently certified NIST standards. Stunner is ready when you are to get validated and make the move into QC.
Stunner’s software hooks labs up with 21 CFR Part 11 compliant features.

Specifications

Stunner instrument specifications

Dimensions37 cm W x 54 cm D x 33 cm H; 26 kg
ElectricalUniversal input voltage 100-240 V AC, 50-60 Hz
ComputerSeparate computer with Windows 10 included
ConnectionUSB, TCP/IP (Service)
ApprovalCE, FCC, CSA
Regulatory complianceOptional 21CFRp11 software package

UV/Vis

Light sourceXenon flash lamp
DetectorsUV/Vis polychromatic spectrophotometer
Wavelength range230-750 nm
Wavelength accuracy

≤400nm: ±1nm; ?400nm: ±2 nm

Spectral resolutionBetter than 2 nm (toluene in hexane)
Absorbance precision (1 cm quartz cuvette)<1 OD: ±0.005 OD st dev1-2 OD: ±0.5% CV
Absorbance accuracy (1 cm quartz cuvette)<1 OD: ±0.01 OD1-2 OD: ±1%

DLS

Light source660 nm laser diode
DetectionAvalanche photodiode module
Size accuracy±2%
Minimum sample concentration0.1 mg/mL lysozyme
Hydrodynamic diameter range0.3–1000nm

Stunner plate specifications

Samples per plate96 (12 x 8 microplate format)
Sample retention timeUp to 2 hours
Recommended sample volume2 µL
Pathlength(s)0.1 mm & 0.7 mm path
Measurement time for full plate~10 minutes for UV/Vis only; ~1 hour for UV/Vis and DLS

Measurement range:

OD 10 mm0.03–275 OD 10 mm
ng/µL dsDNA1.5–13750 ng/µL
mg/mL ave protein mix0.03–275 mg/mL
Absorbance precision (10 mm pathlength)<1 OD: ±0.01 OD st dev


1-200 OD: ±1% CV
Absorbance accuracy (10 mm pathlength)<1 OD: ±0.02 OD


1-200 OD: ±2%

Overview

Stunner is the only system that pulls together UV/Vis concentration, dynamic light scattering (DLS) and static light scattering (SLS) data from the same 2 μL sample.
Dig in to your AAV to get the total capsid titer and empty/full ratio, or rack up payload concentration and size data on any nanoparticle all at once.
Nail down your protein quality by knocking concentration, hydrodynamic size, polydispersity, and detection of aggregates off your list in one shot.
Without skipping a beat, you’ll know if your AAV, nanoparticle or protein is good to go.


AAV capsid titer
AAV empty/full ratio
LNP total RNA quant
Nanoparticle payload quant
Aggregation
Concentration
Sizing & polydispersity
B22 & kD

Capabilities

  • Teeny sample, tons of information

Just load 2 μL of sample in a Stunner plate — don’t bother with sample prep or dilution. Each microfluidic circuit has two fixed pathlengths built into it to cover a wide dynamic range of 0.03-275 OD. If you’re dealing with a full plate, get 96 concentration measurements in 10 minutes — add on DLS sizing and have both done in an hour. For even heavier workflows, hook it up to your favorite robot to add more oomph

  • Look your AAV up and down

Drop in your AAV and before you can blink, DLS & SLS figure out how many intact capsids you have or if a bunch of aggregates are screwing things up. See empty/full ratio, total protein and total ssDNA in about a minute with UV/Vis. Don’t worry about extinction coefficients or overlapping spectra – Stunner does all the math for you.

  • Know your AAV inside out

Get to the numbers you actually want – titers. Stunner bridges DLS and UV/Vis data to tally up how many full and empty capsids are present, and how much extra protein and DNA is left over. Take your cleaned up AAV and sneak a peek down to 1012 vg/mL. In just one assay, Stunner’s dye-free, label-free, standard-free, hassle-free workflow tells the whole titer story.

  • Downstream ready

Smash through the USP & Ph. Eur. UV/Vis requirements for absorbance accuracy, precision, linearity, wavelength accuracy, stray light and resolution with performance verification measurements of independently certified NIST standards. Stunner is ready when you are to get validated and make the move into QC.
Stunner’s software hooks labs up with 21 CFR Part 11 compliant features.

Specifications

Specifications

Stunner instrument specifications

Dimensions37 cm W x 54 cm D x 33 cm H; 26 kg
ElectricalUniversal input voltage 100-240 V AC, 50-60 Hz
ComputerSeparate computer with Windows 10 included
ConnectionUSB, TCP/IP (Service)
ApprovalCE, FCC, CSA
Regulatory complianceOptional 21CFRp11 software package

UV/Vis

Light sourceXenon flash lamp
DetectorsUV/Vis polychromatic spectrophotometer
Wavelength range230-750 nm
Wavelength accuracy?400nm: ±1nm; ?400nm: ±2 nm
Spectral resolutionBetter than 2 nm (toluene in hexane)
Absorbance precision (1 cm quartz cuvette)<1 OD: ±0.005 OD st dev1-2 OD: ±0.5% CV
Absorbance accuracy (1 cm quartz cuvette)<1 OD: ±0.01 OD1-2 OD: ±1%

DLS

Light source660 nm laser diode
DetectionAvalanche photodiode module
Size accuracy±2%
Minimum sample concentration0.1 mg/mL lysozyme
Hydrodynamic diameter range0.3–1000nm

Stunner plate specifications

Samples per plate96 (12 x 8 microplate format)
Sample retention timeUp to 2 hours
Recommended sample volume2 µL
Pathlength(s)0.1 mm & 0.7 mm path
Measurement time for full plate~10 minutes for UV/Vis only; ~1 hour for UV/Vis and DLS

Measurement range:

OD 10 mm0.03–275 OD 10 mm
ng/µL dsDNA1.5–13750 ng/µL
mg/mL ave protein mix0.03–275 mg/mL
Absorbance precision (10 mm pathlength)<1 OD: ±0.01 OD st dev


1-200 OD: ±1% CV
Absorbance accuracy (10 mm pathlength)<1 OD: ±0.02 OD


1-200 OD: ±2%
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