Big Tuna

Overview

  • Cut loose from manual buffer exchange
    Buffer exchange and sample concentration are the time consuming, hands-on chores you have to deal with before all the things you really want to do. Big Tuna automates exchange to formulate, concentrate and clean up proteins or gene therapy vectors like AAVs, LNPs, and VLPs — with just 30 mins of setup time. Skip the slow and manual ways of prepping samples to free yourself up for other critical work.

AAV
LNP
VLP
mRNA & DNA
Protein

  • Capabilities
  • Specifications
  • Go big or keep it small
    Big Tuna serves up two different plate-based formats that let you exchange and concentrate 24 or 96 samples in parallel. Use Unfilter 96 to exchange as little as 100 μL for up to 96 different samples in a run. Unfilter 24 lets you go big and exchange as much as 8 mL on up to 24 samples. With different molecular weight cutoff options, you always have the right Unfilter for the job.
  • Hand it off
    Big Tuna keeps buffer exchange even across the plate with its unique pressure-based UF/DF process and gentle mixing. Its acoustic sensor measures the volume in every well before and after each cycle to track every sample’s exchange rate. Then Big Tuna figures out on the fly how much buffer to add to even things out.
  • Get concentrated
    Dilute samples are a giant pain and hard to handle. Big Tuna can concentrate 48 mL down to 8 mL — freeing you from all the fuss. Follow up with buffer exchange to easily get your AAVs and other low-concentration, high-volume samples right where you need them to be.
  • Be a control freak
    Buffer exchange doesn’t have to be a shot in the dark or keep you anchored to the bench. Use the software wizard to drag and drop your samples and buffers into place, then let Big Tuna guide you through all the ways you can customize the run so it’s just right for any sample type. The easy setup means you’ll have more control over your experiment with way less hands-on time.
  • Know it all
    Load up your samples knowing they’re in good hands. Big Tuna will tell you where to put everything and how much buffer you’ll need. You can check in on progress at any time and know exactly when your exchange and concentration runs will be done. Ditch babysitting your buffer exchange and go do what you really want with your time.

Specifications

Application

Buffer exchange volume rangeUnfilter 24: 0.45-8 mL


Unfilter 96: 100-450 µL

FormulationsUnfilter 24: Up to 24 formulations in parallel


Unfilter 96: Up to 96 formulations in parallel
Sample typesAntibodies and other proteins, nucleic acids, lipid nanoparticles (LNPs), adeno-associated viruses (AAVs), virus-like particles (VLPs)
Exchange time at full volume (96%, at 10 mg/mL IgG)Unfilter 24: 4.5 hours*

)

Unfilter 96: 3 hours*
Protein concentration (range)Up to 200 mg/mL*
Target concentration accuracy± 10%*
Sample recovery

≥96%*

System

Volume measurementUltrasonic sensor
Exchange pressures15, 30 or 60 psi
Operating temperatureRoom temperature
Buffer exchange orbital mixingUnfilter 24: Optimized at 700 rpm


Unfilter 96: Optimized at 875 rpm
Buffer exchange orbital mixingDuty cycle programmable
Physical96 cm L x 81.2 cm D x 130 cm H, 220 kg (instrument)


160 cm L x 100 cm D x 198 cm H, 270 kg (instrument and table)
ElectricalVoltage 100-260 VAC, 50-60 Hz
Nitrogen or CDA requirementPressure 0.55–0.9 MPa (80–130 psi)


Flow rate 40 L/s (85 cfm) minimum

Consumables

Unfilter 2424-well plate, up to 8 mL per well


10, 30, 100 kDa, regenerated cellulose
Unfilter 9696-well plate, up to 450 µL per well


3, 10, 30, 100 kDa, regenerated cellulose
Disposable tips1000 µL non-filtered, automatic re-use up to 12 times per exchange
Dispense precision

<300 µL: ≤3 µL


0.3–8 mL: ≤1%

Overview

  • Cut loose from manual buffer exchange
    Buffer exchange and sample concentration are the time consuming, hands-on chores you have to deal with before all the things you really want to do. Big Tuna automates exchange to formulate, concentrate and clean up proteins or gene therapy vectors like AAVs, LNPs, and VLPs — with just 30 mins of setup time. Skip the slow and manual ways of prepping samples to free yourself up for other critical work.

AAV
LNP
VLP
mRNA & DNA
Protein

Capabilities

  • Go big or keep it small
    Big Tuna serves up two different plate-based formats that let you exchange and concentrate 24 or 96 samples in parallel. Use Unfilter 96 to exchange as little as 100 μL for up to 96 different samples in a run. Unfilter 24 lets you go big and exchange as much as 8 mL on up to 24 samples. With different molecular weight cutoff options, you always have the right Unfilter for the job.
  • Hand it off
    Big Tuna keeps buffer exchange even across the plate with its unique pressure-based UF/DF process and gentle mixing. Its acoustic sensor measures the volume in every well before and after each cycle to track every sample’s exchange rate. Then Big Tuna figures out on the fly how much buffer to add to even things out.
  • Get concentrated
    Dilute samples are a giant pain and hard to handle. Big Tuna can concentrate 48 mL down to 8 mL — freeing you from all the fuss. Follow up with buffer exchange to easily get your AAVs and other low-concentration, high-volume samples right where you need them to be.
  • Be a control freak
    Buffer exchange doesn’t have to be a shot in the dark or keep you anchored to the bench. Use the software wizard to drag and drop your samples and buffers into place, then let Big Tuna guide you through all the ways you can customize the run so it’s just right for any sample type. The easy setup means you’ll have more control over your experiment with way less hands-on time.
  • Know it all
    Load up your samples knowing they’re in good hands. Big Tuna will tell you where to put everything and how much buffer you’ll need. You can check in on progress at any time and know exactly when your exchange and concentration runs will be done. Ditch babysitting your buffer exchange and go do what you really want with your time.

Specifications

Specifications

Application

Buffer exchange volume rangeUnfilter 24: 0.45-8 mL
Buffer exchange volume rangeUnfilter 96: 100-450 ?L
FormulationsUnfilter 24: Up to 24 formulations in parallel
FormulationsUnfilter 96: Up to 96 formulations in parallel
Sample typesAntibodies and other proteins, nucleic acids, lipid nanoparticles (LNPs), adeno-associated viruses (AAVs), virus-like particles (VLPs)
Exchange time at full volume (96%, at 10 mg/mL IgG)Unfilter 24: 4.5 hours*
Exchange time at full volume (96%, at 10 mg/mL IgG)Unfilter 96: 3 hours*
Protein concentration (range)Up to 200 mg/mL*
Target concentration accuracy± 10%*
Sample recovery

≥96%*

System

Volume measurementUltrasonic sensor
Exchange pressures15, 30 or 60 psi
Operating temperatureRoom temperature
Buffer exchange orbital mixingUnfilter 24: Optimized at 700 rpm
Buffer exchange orbital mixingUnfilter 96: Optimized at 875 rpm
Buffer exchange orbital mixingDuty cycle programmable
Physical96 cm L x 81.2 cm D x 130 cm H, 220 kg (instrument)
Physical160 cm L x 100 cm D x 198 cm H, 270 kg (instrument and table)
ElectricalVoltage 100-260 VAC, 50-60 Hz
Nitrogen or CDA requirementPressure 0.55–0.9 MPa (80–130 psi)
Nitrogen or CDA requirementFlow rate 40 L/s (85 cfm) minimum

Consumables

Unfilter 2424-well plate, up to 8 mL per well
Unfilter 2410, 30, 100 kDa, regenerated cellulose
Unfilter 9696-well plate, up to 450 µL per well
Unfilter 963, 10, 30, 100 kDa, regenerated cellulose
Disposable tips1000 µL non-filtered, automatic re-use up to 12 times per exchange
Dispense precision<300 µL: ?3 µL
Dispense precision0.3–8 mL: ?1%
Download Brochure
Know more

Start Your New Experience With Us

Get a Quote !
Get a Quote !