Uncle

Overview

One-stop stability
Cracking stability using a pile of one-trick, protein-hungry tools is a ton of work. Uncle combines 3 different measurement modes — fluorescence, SLS and DLS. So you can crank out all your data in just a few hours, and use way less protein. All the info you’ll get makes picking the best formulation, protein, or viral vector a piece of cake.

Tm & Tagg
Tm with SYPRO (DSF)
Isothermal stability
Sizing & polydispersity
Sizing with thermal ramp
Thermal recovery
Viscosity
kD
B22
G22
ΔG
Viral capsid stability

Capabilities
Specifications

Unleash the Uni
Get more data with way less protein. The Uni only needs 9 μL of sample, and you pick how you use it. Run 1 sample in the morning, 48 in the afternoon. Do a DLS read if that’s all you need. Or, check DLS, then start a 3-day experiment to monitor real-time stability. Your samples are sealed airtight, so runs can be short or long — your call.
Full-spectrum
Biologics behave differently. With Uncle, you get the whole fluorescence spectrum, so you don’t need to know ahead of time how your protein behaves. Uncle catches aggregation with two wavelengths.
Uncover way more in one shot
Trying new formulations or constructs? Get answers for up to 48 samples in under 2 hours. Measure Tm and Tagg at the same time and know when unfolding leads to aggregation. Add a DLS read before the temp ramp to know if you’ve got aggregate trouble right out of the gate.
Isothermal stability
Uncle handles reading DLS, SLS, or both for days with no sample evaporation. So set your temp and walk away. Get a heads-up on long-term stability with the conditions that matter most to you.
Sizing with thermal ramp
Grab polydispersity, diameter, and size distribution with amped sensitivity. Then take the same samples and do a thermal ramp to measure which ones get bent out of shape and which keep their cool.
B22 & kD
Get B22 and kD at the same time in the same Uni. Change up excipients to see if your protein is better off or not — ASAP. Learn on the spot if your protein-formulation combo is good to go or risky for aggregation. For super high protein concentrations, G22 is ready to go.
Capsid stability
Uncle teams up with SYBR Gold to get a read on when your DNA starts to leak — way before the AAV capsid pops.Quantify initial free DNA and the amount that’son the loose after a thermal ramp. Know when aggregation gets out of hand and tackle particle titer with SLS and DLS.

Specifications
Application	Full-spectrum Fluorescence	Static Light Scattering (SLS)	Dynamic Light Scattering (DLS)
Tm	•
Tagg		•
Tm with SYPRO (DSF)	•
Isothermal stability	•	•	•
Sizing & polydispersity			•
Sizing with thermal ramp			•
kD			•
B22 & G22		•
Thermal recovery	•	•
Viscosity			•
ΔG	•
Capsid stability & particle intensity	•	•	•
Instrument
Minimum sample volume	9 µL, sealed capillaries
Simultaneous samples per experiment	48
Sample temperature range	15–95 °C
Sample concentration range	0.05 mg/mL – 300 mg/mL IgG (protein dependent)
Heating rate	0.1–10 °C/minute
Temperature control accuracy	±1 °C (<70 °C), ±1.5 °C (>70 °C)
Physical	54 cm W x 50 cm D x 58 cm H, 50 kg
Electrical	Auto switching power supply, voltage 110–240 V AC, 50–60 Hz, single phase, fuse rating T6.3AL, 250V, max power 600 W
Regulatory compliance	Software has optional 21CFR11
Fluorescence and static light scattering
Sample precision	<2% CV (Tm )
SLS resolution	~15 kDa change in mean molecular mass
AAV genome concentration	≥5 x 10¹¹ viral genomes per mL
Excitation	266 nm and 473 nm laser
Detection	Fluorescence: CCD spectrometer at full 250–720 nm spectral range SLS: intensity at 266 nm and 473 nm
Dynamic light scattering
Hydrodynamic diameter range	0.3–1000 nm
Size accuracy	±2%
Minimum sample concentration	0.1 mg/mL — lysozyme
AAV capsid concentration	≥5 x 10¹¹ viral genomes per mL
Molecular weight range	192 Da – 25 MDa
Light source	660 nm laser diode
Detection	Avalanche photodiode module

Overview

One-stop stability
Cracking stability using a pile of one-trick, protein-hungry tools is a ton of work. Uncle combines 3 different measurement modes — fluorescence, SLS and DLS. So you can crank out all your data in just a few hours, and use way less protein. All the info you’ll get makes picking the best formulation, protein, or viral vector a piece of cake.

Tm & Tagg
Tm with SYPRO (DSF)
Isothermal stability
Sizing & polydispersity
Sizing with thermal ramp
Thermal recovery
Viscosity
kD
B22
G22
ΔG
Viral capsid stability

Capabilities

Unleash the Uni
Get more data with way less protein. The Uni only needs 9 μL of sample, and you pick how you use it. Run 1 sample in the morning, 48 in the afternoon. Do a DLS read if that’s all you need. Or, check DLS, then start a 3-day experiment to monitor real-time stability. Your samples are sealed airtight, so runs can be short or long — your call.

Full-spectrum
Biologics behave differently. With Uncle, you get the whole fluorescence spectrum, so you don’t need to know ahead of time how your protein behaves. Uncle catches aggregation with two wavelengths.

Uncover way more in one shot
Trying new formulations or constructs? Get answers for up to 48 samples in under 2 hours. Measure Tm and Tagg at the same time and know when unfolding leads to aggregation. Add a DLS read before the temp ramp to know if you’ve got aggregate trouble right out of the gate.
Isothermal stability
Uncle handles reading DLS, SLS, or both for days with no sample evaporation. So set your temp and walk away. Get a heads-up on long-term stability with the conditions that matter most to you.
Sizing with thermal ramp
Grab polydispersity, diameter, and size distribution with amped sensitivity. Then take the same samples and do a thermal ramp to measure which ones get bent out of shape and which keep their cool.
B22 & kD
Get B22 and kD at the same time in the same Uni. Change up excipients to see if your protein is better off or not — ASAP. Learn on the spot if your protein-formulation combo is good to go or risky for aggregation. For super high protein concentrations, G22 is ready to go.
Capsid stability
Uncle teams up with SYBR Gold to get a read on when your DNA starts to leak — way before the AAV capsid pops.Quantify initial free DNA and the amount that’son the loose after a thermal ramp. Know when aggregation gets out of hand and tackle particle titer with SLS and DLS.

Specifications

Specifications
Application	Full-spectrum Fluorescence	Static Light Scattering (SLS)	Dynamic Light Scattering (DLS)
Tm	•
Tagg		•
Tm with SYPRO (DSF)	•
Isothermal stability	•	•	•
Sizing & polydispersity			•
Sizing with thermal ramp			•
kD			•
B22 & G22		•
Thermal recovery	•	•
Viscosity			•
ΔG	•
Capsid stability & particle intensity	•	•	•
Instrument
Minimum sample volume	9 µL, sealed capillaries
Simultaneous samples per experiment	48
Sample temperature range	15–95 °C
Sample concentration range	0.05 mg/mL – 300 mg/mL IgG (protein dependent)
Heating rate	0.1–10 °C/minute
Temperature control accuracy	±1 °C (<70 °C), ±1.5 °C (>70 °C)
Physical	54 cm W x 50 cm D x 58 cm H, 50 kg
Electrical	Auto switching power supply, voltage 110–240 V AC, 50–60 Hz, single phase, fuse rating T6.3AL, 250V, max power 600 W
Regulatory compliance	Software has optional 21CFR11
Fluorescence and static light scattering
Sample precision	<2% CV ™
SLS resolution	~15 kDa change in mean molecular mass
AAV genome concentration	?5 x 1011 viral genomes per mL
Excitation	266 nm and 473 nm laser
Detection	Fluorescence: CCD spectrometer at full 250–720 nm spectral range SLS: intensity at 266 nm and 473 nm
Dynamic light scattering
Hydrodynamic diameter range	0.3–1000 nm
Size accuracy	±2%
Minimum sample concentration	0.1 mg/mL — lysozyme
AAV capsid concentration	?5 x 1011 viral genomes per mL
Molecular weight range	192 Da – 25 MDa
Light source	660 nm laser diode
Detection	Avalanche photodiode module

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